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Developments in the
19th Century:
"Carl
Zeiss + Ernest Abbe + Otto Schott = Perfection"
Zeiss:
Son of a toy maker
Apprenticed for 12 years in
scientific workshops
Went into business in 1846
as an instrument maker
Guided into making
microscopes by the founder of modern botany, Jakob Schleiden
Turned out his 1000th
microscope in 1866
Became unhappy with
trial-and-error methods.
Teamed up with a young
physicist, Ernest Abbe
Abbe:
Abbe worked with Zeiss to
discover that the problem with existing microscopes was that rays from
different parts of the lens came to focus at different places (spherical
aberration).
Developed exact mathematical
formulas for the shapes, sizes, and positions of lenses that would solve
this problem.
Built 12 microscopes to
these specifications. All were inferior to the trial-and-error
versions.
At Zeiss' persistance, he
spent the next two years on the problem, working out new specifications
based on the "new" wave theory of light. The result was
the 'Abbe Sine Condition,' still used today as the basis for designing
microscopes.
These new microscopes were a
success! They outperformed those of the best English and French
versions, and could be made in a repeatable manner.
As it turned out, all of the
"trial-and-error" microscopes made previously ALSO fulfilled
the 'Abbe Sine Condition' but for no known reasons, other than they
achieved the best performance!
These
"theoretical" microscopes still suffered from chromatic
aberration, the color fringing in the images. All they had
available were two glass types--crown and flint.
Schott:
A young chemist who wrote
his thesis on the manufacture of window glass.
Interested in finding new
kinds of glass.
Joined Zeiss and Abbe in
1884 (The Technical Glass Laboratory of Schott and Co.)
Two years later they issued
a catalog of 44 different types of glass, including those needed for
better color-correction in the microscope.
Footnote:
In 1889, just after the
death of Carl Zeiss, Abbe created the "ultimate"
microscope. It featured an oil-immersion type objective lens, the
famous 'Abbe' condenser lens to illuminate the specimen, and a newly
designed eyepiece. Combined, this microscope provided the ultimate
in resolving power of 1/2 the wavelength of light used, as shown by
Abbe's own theory (and which remains the ultimate in resolving power to
this day).
Provided useful
magnifications of up to 2000X.
Immediately used to identify
"good" from "bad" bacteria, which were a few
wavelengths across.
It would take the advent of
the electron microscope to identify viruses, however.
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