Multiphoton Microscope Driven by Hand-Held 1550-NM Laser

    Assistant professor Khanh Kieu recently developed a multiphoton microscope that is based on a turn-key, handheld 150-femtosecond laser that he created during graduate studies at OSC. This microscope uses a fundamental wavelength of 1550 nanometers to study samples that include thin film materials such as graphene, biological samples such as diatoms and nanophotonic devices such as electro-optic polymer/silicon hybrid modulators.
     
    The picture to the right shows the multiphoton imaging of a diatom, a species of plankton. The red region indicates second harmonic light at 775 nm, while the green regions correspond to third harmonic generation at 517 nm. The image reveals the microstructure of the diatom, assisting researchers in better understanding photosynthesis and other processes in these pervasive organisms. This work points toward using multiphoton microscopy to interrogate a wide range of biological specimens, as well as micro- and nanophotonics circuits.
    Marine Diatom image
    Multiphoton microscope image of a marine diatom.