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TITLE
Analyzing neuron computing with two-photon 3D imaging at 100Hz
ABSTRACT
How neurons receive, integrate, and transmit information lies at the heart of understanding how the brain computes. Capturing these events therefore requires an imaging system that can see sub-micron fine structures, follow rapid changes, and cover an extended 3D volume at the same time.
In this talk, Leilei Peng will present the dual-view Bessel two-photon projection microscopy (dv-B2PM), which bridges the need for high imaging speed and synaptic-level volumetric resolution. The system captured rapid calcium activity across extended neuronal structures while maintaining sub-micron spatial resolution. By pairing dv-B2PM with localized two-photon glutamate uncaging, we observed fast and continuous spatiotemporal Ca2+ activities extending from the apical dendrite to the soma.
Through multi-timescale spatial-temporal analysis of the 3D timelapse image data, rapid and localized events over the entire neuron were detected in unprecedented resolution and speed. These results likely underlie the mechanisms of dendritic plateau potential generation and signal summation that shape neuronal computation. dv-B2PM provides a powerful framework for exploring circuit-level integration and an advanced all-optical platform for dissecting the principles of neuronal integration.
BIO
Leilei Peng received her Ph.D. in Physics from Purdue University in 2005. She then completed postdoctoral training at the Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, where she was promoted to Instructor in 2008 and launched her independent research program. In 2009, she joined the Wyant College of Optical Sciences at the University of Arizona. She also served as a Visiting Professor at Stanford Medicine from 2017 to 2018.
Dr. Peng's research focuses on the invention and development of advanced fluorescence imaging technologies for biomedical applications. Her laboratory specializes in designing and building innovative optical imaging instruments and works closely with collaborators in the biological and biomedical sciences to develop cutting-edge approaches for deep-tissue imaging, multiplexed fluorescence microscopy, and functional volumetric microscopy.